Description of cell adhesion by the langmuir adsorption isotherm.
نویسندگان
چکیده
The adhesion of granulocytes to a glass surface is both theoretically and experimentally investigated. The basic results are: (i) The adherence process of cells to a surface can be described by the Langmuir adsorption isotherm which also holds for the adsorption of molecules to a substrate. (ii) The granulocytes do not interact with each other during the adsorption/desorption process. (iii) The equilibrium constant at 37 degrees C for granulocytes adsorption to the glass surface is 170 cells/mm3 = 1.7 x 10(5) cells/ml. (iv) The equilibrium constant increases with decreasing temperature. (v) The equilibrium constant is a function of the chemokinetic/chemotactic stimulus f-Met-Leu-Phe. (vi) The desorption rate is also a function of the chemokinetic/chemotactic stimulus (kde-1 = 71.5 min for 1 nM and 330 min for 1 microM f-Met-Leu-Phe). (vii) The adsorption rate is a function of the chemokinetic/chemotactic stimulus and the cellular bulk concentration (kad-1.cv-1 = 104 min for 1 nM and 8 min for 1 microM f-Met-Leu-Phe, and cV = 4000 cells/mm3 = 4 x 10(6) cells/ml). (viii) The total number of cells which can bind to the surface is independent of temperature (and of the chemokinetic/chemotactic stimulus). (ix) The high affinity receptor site is responsible for the adherence (Kf-Met- = 1.8 nM f-Met-Leu-Phe). (x) The chemokinetic/chemotactic stimulated adherence is a cooperative process on a molecular level. (xi) The cellular adsorption/desorption process is a rate controlled process. The thermodynamic description of the adsorption/desorption process failed.
منابع مشابه
Kinetic Study and Equilibrium Isotherm Analysis of Nickel (II) Adsorption onto Alginate-SBA-15 Nanocomposite
The present work was undertaken to evaluate the feasibility of nickel (II) removal from aqueous solution by adsorption onto a biopolymer adsorbent. The adsorbent was prepared using modification of mesoporous silicate SBA-15 with alginate biopolymer (alginateSBA-15) by encapsulation method. Morphological structure of the obtained nanocomposite adsorbent was characterized by XRD, scanning electro...
متن کاملInvestigation of Langmuir and Freundlich Adsorption Isotherm of Pb2+ Ions by Micro Powder of Cedar Leaf
ABSTRACTIn this work, the micro powder was the product of cedar leaf (MPCL) is used as a low-cost adsorbent for the removal of Pb2+ ions from aqueous solutions. Bath experiments were used to determine the best adsorption conditions The adsorption percentage of Pb2+ ions MPCL samples at different initial pH, contact time (tc), adsorbent dosage and temperature (T) were investigated. Effective rem...
متن کاملEnhanced removal of Bismark Brown(BB) dye from aqueous solutions using activated carbon from raw Ziziphusspina-christi (ZSAC) Equilibrium, thermodynamic and kinetics
In this study, carbon was easily made from walnut wood as a low-cost and non-toxic natural adsorbent. Ziziphusspina-christi (ZSAC), an excellent adsorbent, were successfully synthesized by an in chemical vapor deposition method and used for the removal of Bismark Brown(BB) dye from aqueous solution. The as-synthesized Ziziphusspina-christi (ZSAC) were characterized by FT-IR technique. Various o...
متن کاملA modified Langmuir-Freundlich isotherm model for simulating pH-dependent adsorption effects.
Analytical isotherm equations such as Langmuir and Freundlich isotherms are widely used for modeling adsorption data. However, these isotherms are primarily useful for simulating data collected at a fixed pH value and cannot be easily adapted to simulate pH-dependent adsorption effects. Therefore, most adsorption studies currently use numerical surface-complexation models (SCMs), which are more...
متن کاملModeling of Experimental Adsorption Isotherm Data
Adsorption is considered to be one of the most effective technologies widely used in global environmental protection areas. Modeling of experimental adsorption isotherm data is an essential way for predicting the mechanisms of adsorption, which will lead to an improvement in the area of adsorption science. In this paper, we employed three isotherm models, namely: Langmuir, Freundlich, and Dubin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. C, Journal of biosciences
دوره 43 9-10 شماره
صفحات -
تاریخ انتشار 1988